Effect of safflower yellow on atherosclerosis in rabbits with hyperlipidemia / 国际药学研究杂志

Objective To investigate the effect of safflower yellow injection on atherosclerosis in rabbits with hyperlipidemia.Methods Ninety-six New Zealand rabbits were randomly divided into four groups:the control group, model group, safflower yellowtest group (10.9, 5.45 and 2.725 mg/kg) and the positive control (atorvastatin) group. The control group was fed with normal feed, while the other three groups were fed with high fat diet for 8 weeks, combined with intraperitoneal injection of vitamin D3, to establish hyperlipidemia model. Then, the three-dosage safflower yellow-test groups were given intraperitoneal injection of safflower yellow (10.9, 5.45 and 2.725 mg/kg), respectively, the positive control group was given atorvastatin calcium[2 mg/ (kg·d) ]by intragastric administration, and the control and model groups were orally given an equal volume of normal saline, all once a day every day for 8weeks. After 16 h fasting following the last administration, the body weight, total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), oxidized low density lipoprotein (OX-LDL), matrix metalloproteinases 9 (MMP-9), tissue inhibitors of metalloproteinase 1 (TIMP-1), apolipoprotein E (ApoE), low density lipoprotein receptor (LDL-R), and scavenger receptor class B type1 (SR-B1) levels were measured. The morphological changes of thoracic aortas were examined by HE staining. Results At the 16 th week, compared with the control group, the body weight as well as the TC, TG, LDL-C, OX-LDL, MMP-9, HIF-1α, VEGF, VCAM-1 and PF4 level were all increased significantly (P＜0.05), while the level of HDL-C, ApoE, LDL-R, and SR-B1 decreased significantly (P＜0.05), accompanied with the atherosclerotic changes in the thoracic aortas indicated by the HE staining in the model group. Compared with the model group, the body weight as well as the TC, LDL-C, OX-LDL, MMP-9, HIF-1α, VEGF, VCAM-1 and PF4 level were decreased significantly (P＜0.05), and the TIMP-1 level increased (P＜0.05) in all of the three-dosage safflower yellow-test groups. Meanwhile, compared with the model group, in the 10.9 and 5.45 mg/kg safflower yellow groups, the TG level were decreased and the ApoE and SR-B1 levels were increased significantly (P＜0.05). On the other hand, the LDL-R level significantly increased only in the safflower yellow 10.9 mg/kg group (P＜0.05). HE staining showed a significant reduction in atherosclerorotic changes of the thoracic aorta in the safflower yellow-test groups. Conclusion Safflower yellow may inhibit the progression of atherosclerosis by regulating the lipid metabolism and MMP-9/TIMP-1 balance and also by inhibiting the HIF-1α, VEGF, VCAM-1 and PF4 expression.

Association between Ambient Air Pollution and Hospital Emergency Admissions for Respiratory and Cardiovascular Diseases in Beijing: a Time Series Study / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To investigate the association between ambient air pollution and hospital emergency admissions in Beijing.</p><p><b>METHODS</b>In this study, a semi-parametric generalized additive model (GAM) was used to evaluate the specific influences of air pollutants (PM10, SO2, and NO2) on hospital emergency admissions with different lag structures from 2009 to 2011, the sex and age specific influences of air pollution and the modifying effect of seasons on air pollution to analyze the possible interaction.</p><p><b>RESULTS</b>It was found that a 10 μg/m3 increase in concentration of PM10 at lag 03 day, SO2 and NO2 at lag 0 day were associated with an increase of 0.88%, 0.76%, and 1.82% respectively in overall emergency admissions. A 10 μg/m3 increase in concentration of PM10, SO2 and NO2 at lag 5 day were associated with an increase of 1.39%, 1.56%, and 1.18% respectively in cardiovascular disease emergency admissions. For lag 02, a 10 μg/m3 increase in concentration of PM10, SO2 and NO2 were associated with 1.72%, 1.34%, and 2.57% increases respectively in respiratory disease emergency admissions.</p><p><b>CONCLUSION</b>This study further confirmed that short-term exposure to ambient air pollution was associated with increased risk of hospital emergency admissions in Beijing.</p>

Inhibiting Smooth Muscle Cell Proliferation via Immobilization of Heparin/Fibronectin Complexes on Titanium Surfaces / 生物医学与环境科学（英文）

The aim of this study was to investigate the inhibitory effect of heparin/fibronectin (Hep/Fn) complexes on neointimal hyperplasia following endovascular intervention. Hep/Fn complexes were immobilized onto titanium (Ti) surfaces, with subsequent X-ray photoelectron spectroscopy (XPS), Toluidine Blue O (TBO) and immunohistochemistry methods were used to characterize surface properties. Smooth muscle cell (SMC) cultures were used to evaluate the effect of Hep/Fn complexes on SMC proliferation. Results showed that Hep/Fn complexes successfully immobilized onto Ti surfaces and resulted in an inhibition of SMC proliferation. This study suggests that Hep/Fn surface-immobilized biomaterials develop as a new generation of biomaterials to prevent neointimal hyperplasia, particularly for use in cardiovascular implants.

Analysis of young male anthropometric parameters for design of driving and operations in cars / 生物医学工程学杂志

Fitting formulas of typical parameters were obtained through analysis of anthropometric parameters of armored car driver and operators. These formulas could be a basis for design of product and equipment of cars, cumulating and updating of basic data of similar sample and edition of relative standards. 76 anthropometric static parameters and 11 functional parameters were chosen, and 1 243 soldiers with armored forces were chosen to be tested. The correlation and fitting formulas of body height, sitting height and other parameters were measured and obtained. We also contrasted measured data with data from GJB1835-1993. The present analysis showed that the correlation between sizes of body length and body height and sitting height was significant. Sizes of body length and enclose size and width direction were all increased compared to those in the 1980s. The present results were consistent with other researchers' current research results. The measured data could be an important basis for the data of young male anthropometric parameters and edition of relative standards and design of specific equipment.

Effects of Stainless Steel and Titanium Oxide in Stimulation of Endothelial Cells Releasing NO and MCP-1 / 现代生物医学进展

To investigate the influence of stainless steel(SS)and titanium oxide(Ti-O)thin films on the cytokine released by en-dothelial cells.Ti-O films were prepared by unbalanced magnetron sputtering deposition system(UBMS).Human umbilical vein en-dothelial cells(HUVECS)were isolated and seeded on SS and Ti-O materials.Nitride oxide (NO)and monocytc chemotactic protein one(MCP-1)concentrations were determined in the supematants with enzyme linked immunosorbent assay(ELISA)at 1,3 and 5 days after culture,and the morphology of the cells was observed using scanning electron microscopy(SEM).The results showed that the similar amount of HUVECs was presented in SS and Ti-O culture.But HUVEC had better morphology on Ti-O film than SS.The NO and MCP.1 test also showed that HUVECs on Ti-O film had a better bio-function than SS.It is concluded that Ti-O film as a biomaterial may have a better endothlieal compatibility,which can be used to enhance the endothelialization.

Effects of Stainless Steel and Titanium Oxide in Stimulation of Endothelial Cells Releasing NO and MCP-1 / 现代生物医学进展

To investigate the influence of stainless steel(SS)and titanium oxide(Ti-O)thin films on the cytokine released by en-dothelial cells.Ti-O films were prepared by unbalanced magnetron sputtering deposition system(UBMS).Human umbilical vein en-dothelial cells(HUVECS)were isolated and seeded on SS and Ti-O materials.Nitride oxide (NO)and monocytc chemotactic protein one(MCP-1)concentrations were determined in the supematants with enzyme linked immunosorbent assay(ELISA)at 1,3 and 5 days after culture,and the morphology of the cells was observed using scanning electron microscopy(SEM).The results showed that the similar amount of HUVECs was presented in SS and Ti-O culture.But HUVEC had better morphology on Ti-O film than SS.The NO and MCP.1 test also showed that HUVECs on Ti-O film had a better bio-function than SS.It is concluded that Ti-O film as a biomaterial may have a better endothlieal compatibility,which can be used to enhance the endothelialization.

The curative effect of type 2 diabetes complicated with pulmonary tuberculosis by rosiglitazone combined with insulin / 中国基层医药

0.05);there were significant decrease in FPG,PPG and HbA1c in the DM+INS+RSG group after 1 and 3 months treatment(P

Application of approximate entropy and complexity analysis in monitoring depth of anesthesia / 解放军医学杂志

Objective The present study was undertaken to investigate the properties of nonlinear dynamics of EEG and the changes in depth of anesthesia with real-time approximate entropy (ApEn) and complexity (Cx) nonlinear indexes monitoring during anesthesia. Methods EEG was recorded in 65 in-patients. They were randomly divided into 4 groups: isoflurane, sevoflurane, desflurane (n=15, respectively), and propofol intravenous anesthesia (n=20) groups. The EEG derived parameters ApEn and Cx non-linear indexes were calculated simultaneously during the whole operation including rest state with eyes closed, anesthetic induction, intraoperation, recovery, post-operation awaking. Results ApEn and Cx nonlinear indexes remained the highest during rest state. Both of them kept decreasing during anesthetic induction. They dropped to a relative lower value and leveled off in the intra-operation period. Both of them rose gradually during recovery period and returned to a high level in the post-operation awaking period (correspondingly, ApEn: 0.87, 0.78, 0.55, 0.64 and 0.83. Cx: 0.58, 0.54, 0.38, 0.46 and 0.57). Conclusions With ApEn and Cx non-linear indexes, changes in depth of anesthesia from EEG signal could be real-timely monitored and precisely measured. Nonlinear dynamic analysis might provide us with more information about consciousness and cognition during general anesthesia.